Intracellular Total ROS Activity BioAssay™ Kit

Cat# 474028-100T

Size : 100Tests

Brand : US Biological



474028 Intracellular Total ROS Activity BioAssay™ Kit

Clone Type
Polyclonal
Applications
FC
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sample Type:|Cell culture||Target:|ROS||Excitation/Emission:|490nm/520nm||Method of Analysis:|Flow Cytometry||Intended Use:|Intracellular Total ROS BioAssay™ Assay provides a good screening option for assessing the potency of oxidative stress inhibitor and activator reagents, and will help to determine how oxidative stress modulates varied intracellular pathways. This product assesses the overall level of intracellular ROS activity and can be analyzed using a flow cytometry.||Test Principle:|Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer, has been well established.||Intracellular Total ROS Activity BioAssay™ provides a good screening option for assessing the potency of oxidative stress inhibitor and activator reagents, and will help to determine how oxidative stress modulates varied intracellular pathways. This kit assesses the overall level of intracellular ROS activity, but does not identify the specific reactive oxygen molecule(s) generated by the oxidative stress event.||The key reagent in the BioAssay™ is a proprietary dye called Total ROS Green. This dye quickly penetrates membrane structures and accumulates within the cell. In the presence of ROS, the non-fluorescent Total ROS Green dye molecule is oxidized by all various iterations of ROS molecular forms. In the oxidized state, the Total ROS Green dye molecule acquires fluorescence properties that enable its detection by flow cytometry as an indicator of the relative level of intracellular ROS activity.||Kit Components:|Total ROS Green, lyophilized, 1 vial|DMSO, 1x200uL, 1 vial|BioAssay™ Buffer, 10X, 1x15mL||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Hoffmann, O. M., Becker, D., and Weber, J. R. (2007) J Cereb Blood|Flow Metab.|2. Funk, R. S., and Krise, J. P. (2007) Mol Pharm.|3. Krebs, B., Wiebelitz, A., Balitzki-Korte, B., Vassallo, N., Paluch, S., Mitteregger, G., Onodera, T., Kretzschmar, H. A., and Herms, J. (2007) J Neurochem.|4. Yang, Y., Xu, S., An, L., and Chen, N. (2007) J Plant Physiol.|5. Lee, J. E., Kim, H., Jang, H., Cho, E. J., and Youn, H. D. (2007) J Neurochem

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Cat#
Description
Cond.
Price Bef. VAT
KA3841
 1Kit