Rat PDGFRa (Platelet Derived Growth Factor Receptor Alpha) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Pdgfra
Gene ID:	25267
Uniprot ID:	PGFRA_RAT
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity
Post Translational Modifications	Ubiquitinated, leading to its internalization and degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-730 and Tyr-741 is important for interaction with PIK3R1. Phosphorylation at Tyr-719 and Tyr-753 is important for interaction with PTPN11. Phosphorylation at Tyr-761 is important for interaction with CRK. Phosphorylation at Tyr-571 and Tyr-573 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-987 and Tyr-1017 is important for interaction with PLCG1.
Function	Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Protein Name	Platelet-Derived Growth Factor Receptor Alpha Pdgf-R-Alpha Pdgfr-Alpha Alpha Platelet-Derived Growth Factor Receptor Alpha-Type Platelet-Derived Growth Factor Receptor Cd140 Antigen-Like Family Member A Platelet-Derived Growth Factor Alpha Receptor Cd Antigen Cd140a
Database Links	Reactome: R-RNO-1257604 Reactome: -RNO-186763 Reactome: -RNO-186797 Reactome: -RNO-5673001 Reactome: -RNO-6811558
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Cilium Golgi Apparatus
Alternative ELISA Names	Platelet-Derived Growth Factor Receptor Alpha ELISA kit Pdgf-R-Alpha ELISA kit Pdgfr-Alpha ELISA kit Alpha Platelet-Derived Growth Factor Receptor ELISA kit Alpha-Type Platelet-Derived Growth Factor Receptor ELISA kit Cd140 Antigen-Like Family Member A ELISA kit Platelet-Derived Growth Factor Alpha Receptor ELISA kit Cd Antigen Cd140a ELISA kit Pdgfra ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance