Reagents and instruments for immunology, cell biology and molecular biology.

Anti-AP-2 Alpha/Beta antibody (360-440 C-Term)

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Cat# NB-22-1617-200ul

Size : 200µg

Brand : Neo Biotech

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using AP-2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO205 cells, using AP-2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using AP-2 Alpha/Beta Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Transcription factor AP-2-alpha and Transcription factor AP-2-beta (388-437 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	TFAP2B TFAP2A
Gene ID:	7021 7020
Uniprot ID:	AP2B_HUMAN AP2A_HUMAN
Immunogen Region:	388-437 aa
Specificity:	AP-2 Alpha/Beta Polyclonal Antibody detects endogenous levels of AP-2 Alpha/Beta protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human AP-2 at the amino acid range 388-437

Description

Function	Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Protein Name	Transcription Factor Ap-2-Alpha Ap2-Alpha Ap-2 Transcription Factor Activating Enhancer-Binding Protein 2-Alpha Activator Protein 2 Ap-2
Database Links	Reactome: R-HSA-3232118 P05549-1 Reactome: R-HSA-8864260 Reactome: R-HSA-8866904 Reactome: R-HSA-8866906 Reactome: R-HSA-8866907 Reactome: R-HSA-8866910 Reactome: R-HSA-8866911 Reactome: R-HSA-8869496
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-Transcription Factor Ap-2-Alpha antibody Anti-Ap2-Alpha antibody Anti-Ap-2 Transcription Factor antibody Anti-Activating Enhancer-Binding Protein 2-Alpha antibody Anti-Activator Protein 2 antibody Anti-Ap-2 antibody Anti-TFAP2A antibody Anti-AP2TF antibody Anti-TFAP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance