Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CD5 (PE) Monoclonal Antibody

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Cat# 227430-100T

Size : 100Tests

Brand : US Biological

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227430 CD5 (PE)

Clone Type

Polyclonal

Host

mouse

Source

equine

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Recognizes the equine CD5 antigen, a single-pass type I membrane protein with a molecular weight of ~69kD. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CVS5|Host: mouse|Source: equine|Concentration: Not determined |Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein G affinity chromatography.|Immunogen: Equine leucocytes.|Specificity: Recognizes equine CD5.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Equine leucocytes.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes equine CD5.

References

1. Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunology 74: 251-257. 2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 3. Moyo, N.A. et al. (2013) Differentiation and activation of equine monocyte-derived dendritic cells is not correlated with CD206 or CD83 expression. Immunology. Mar 5. [Epub ahead of print]. 4. Mayall, S. et al. (2001) The anti-human CD21 antibody, BU33, identifies equine B cells. J Comp Pathol. 124: 83-7. 5. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells. Vet Immunol Immunopathol. 60: 15-31.