Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-B9) - Biotin

SARSCoV2 Nucleocapsid (N) Protein

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This biotinylated antibody is stable when stored at 28°C. Do not freeze.

Ships Overnight on Blue Ice

AntiSARSCoV2 Nucleocapsid, clone NP1B9, specifically targets an epitope on the SARSCoV2 nucleocapsid protein. Futhermore, it is reported to bind to the oligomerization domain of the N protein.

Severe acute respiratory syndrome coronavirus 2 (SARSCoV2), the causative agent of coronavirus disease 2019 (COVID19), is an enveloped, singlestranded, positivesense RNA virus belonging to the Coronaviridae family¹. The SARSCoV2 genome encodes four essential proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins². SARSCoV2 shares 79.6% identity with the original SARSCoV². The N protein is 46 kDa and consists of two highly conserved structural domains, the Nterminal domain (NTD) and Cterminal domain (CTD), connected by a linker region. The NTD and CTD are involved in a couple of primary functions, including RNA binding and selfoligomerization^3,4. This results in binding to and packaging of the viral RNA genome into a helical ribonucleoprotein⁵. The N protein is involved in other critical steps of the viral life cycle, including transcription, replication, and modulating infected cell signaling pathways^6,7. The N protein is a suitable candidate for vaccine development and diagnostic assays⁸ for several reasons. It is abundantly expressed during infection and is highly conserved, sharing 90% amino acid homology with the SARSCoV N protein⁹. Furthermore, antibodies^9,10 and memory T cells^11,12 targeting the N protein are identified in the sera of convalescent COVID19 patients, demonstrating it as immunogenic. The N protein also suppresses antiviral RNAi, evading the innate immune system¹³, suggesting its potential value as a targeted therapeutic.

The nucleocapsid protein is expressed in the internal nucleocapsid of SARSCoV2.

1. Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020.
2. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020.
3. Kang S, Yang M, Hong Z, et al. Acta Pharm Sin B. 10.1016/j.apsb.2020.04.009. 2020.
4. Chang CK, Sue SC, Yu TH, et al. J Biomed Sci. 13(1):5972. 2006.
5. Hsieh PK, Chang SC, Huang CC, et al. J Virol. 79(22):1384813855. 2005.
6. Surjit M, Lal SK. Infect Genet Evol. 8(4):397405. 2008.
7. Hurst KR, Ye R, Goebel SJ, Jayaraman P, Masters PS. J Virol. 84(19):1027610288. 2010.
8. Liu L, Liu W, Zheng Y, et al. Microbes Infect. 22(45):206211. 2020.
9. Guo L., Ren L., Yang S., et al. Clinical Infectious Diseases: an Official Publication of the Infectious Diseases Society of America. 2020.
10. To K.K., Tsang O.T., Leung W.S., et al. Lancet Infect. Dis. 2020.
11. Grifoni A., Weiskopf D., Ramirez S.I., et al. Cell. 2020.
12. Ni L, Ye F, Cheng ML, et al. Immunity. 52(6):971977.e3. 2020.
13. Mu J, Xu J, Zhang L, et al. Sci China Life Sci. 14. 2020.