Hygromycin B [31282-04-9]
Cat# HY-B0490-5g
Size : 5g
Brand : MedChemExpress
Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.
For research use only. We do not sell to patients.
Hygromycin B Chemical Structure
CAS No. : 31282-04-9
This product is a controlled substance and not for sale in your territory.
Based on 18 publication(s) in Google Scholar
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Description |
Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells. |
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IC50 & Target |
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In Vitro |
Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
527.52 |
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Formula |
C20H37N3O13 |
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CAS No. |
31282-04-9 |
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Appearance |
Solid |
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Color |
White to light yellow |
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SMILES |
O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)(19)[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)(19)[C@@H]([C@H]4NC)O)(19)[C@@]([C@H]3O)(19)O2 |
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Structure Classification |
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Initial Source |
Escherichia coli |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 100 mg/mL (189.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 50 mg/mL (94.78 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: ≥98.0% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 1.8957 mL | 9.4783 mL | 18.9566 mL | 47.3916 mL |
5 mM | 0.3791 mL | 1.8957 mL | 3.7913 mL | 9.4783 mL | |
10 mM | 0.1896 mL | 0.9478 mL | 1.8957 mL | 4.7392 mL | |
15 mM | 0.1264 mL | 0.6319 mL | 1.2638 mL | 3.1594 mL | |
20 mM | 0.0948 mL | 0.4739 mL | 0.9478 mL | 2.3696 mL | |
25 mM | 0.0758 mL | 0.3791 mL | 0.7583 mL | 1.8957 mL | |
30 mM | 0.0632 mL | 0.3159 mL | 0.6319 mL | 1.5797 mL | |
40 mM | 0.0474 mL | 0.2370 mL | 0.4739 mL | 1.1848 mL | |
50 mM | 0.0379 mL | 0.1896 mL | 0.3791 mL | 0.9478 mL | |
60 mM | 0.0316 mL | 0.1580 mL | 0.3159 mL | 0.7899 mL | |
80 mM | 0.0237 mL | 0.1185 mL | 0.2370 mL | 0.5924 mL | |
DMSO | 100 mM | 0.0190 mL | 0.0948 mL | 0.1896 mL | 0.4739 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.