Biomarker Responses in the Freshwater Shrimp Caridina nilotica as Indicators of Persistent Pollutant Exposure
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P450 Demethylation Fluorescent Activity Kit
Size : 2x96wellplate
The DetectX® P450 Demethylation Fluorescent Activity Kit provides a reliable method for quantitatively measuring demethylating P450 system activity. Suitable for any P450 system or biological system producing Formaldehyde as a demethylation product, this kit features a 45 to 90 minute run time. Detailed instructions are available in the kit insert. Please read instructions thoroughly before starting the assay. The kit includes a Formaldehyde Standard for generating a precise standard curve.
Cytochrome P450 enzymes, present in most tissues, play important roles in various biological functions such as hormone synthesis and breakdown, cholesterol synthesis, and vitamin D metabolism. They are also key players in drug metabolism, influencing the development of new pharmaceuticals and drug interactions. As a superfamily of heme-containing enzymes, cytochrome P450s exhibit significant diversity in substrate specificity and catalytic activity.
These enzymes metabolize a wide range of exogenous and endogenous compounds, participating in multicomponent electron transfer reactions. In toxicology, cytochrome P450 enzymes are of particular interest as they break down toxins, preventing harmful accumulation in the body. Their involvement extends to the metabolism of environmental chemicals and carcinogens and determines the sensitivity of individuals to various environmental toxins and drugs.
The DetectX® P450 Demethylation Fluorescent Activity Kit provides a sensitive and straightforward approach to assessing the activity of these enzymes, facilitating research in drug development, toxicology, and understanding the enzymatic mechanisms underpinning critical physiological processes.