Protein A SuperSpin
Cat# 280301
Size : 50/pack
Brand : Biotoolomics
Introduction
Protein A SuperSpin is supplied for fast small-scale purification of immunoglobulin G (IgG) from various species (e.g. serum, ascites and mammalian cell culture broth etc). Protein A has been well proven a powerful affinity ligand which shows very high specificity to IgGs from mammalian hosts.
Each Protein A SuperSpin tube accommodates 50 µl of Protein A agarose particles. The affinity resin is specifically designed to provide large pore size with small particle size (i.e. 20 – 50 µm). So large antibody molecules can access the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantity of antibody can be easily purified within 20 minutes with inexpensive cost. No special expensive chromatography instrument and column is required.
- Protein A SuperSpin is particularly useful for the following applications:
- The quantity of antibody to be purified is small (less than half milligram)
- On-line detection of antibody expression level
- High throughput purification of antibodies
- High throughput screening of antibodies
- Key benefits:
- Rapid antibody capture and recovery
- High antibody binding capacity (half milligram)
- High throughput format (24 samples can be processed in parallel)
- Low cost
- Easy to use and no expensive equipment required
Characteristics of Ni SuperSpin / Cu SuperSpin / Co SuperSpin / Zn SuperSpin
Tube material | Polypropylene |
Resin | Protein A SepFast |
Particle size | 20 – 50 µm |
Packed volume | 50 µl |
Protein binding capacity | Depends on the type of proteins and binding conditions; up to 500 µg |
Chemical compatibility | The resin maintains the binding capability after exposing to 3M NaSCN, 6M GuHCl or 8M urea over a few hours |
Storage condition | 2oC – 8oC |