Protein A SuperSpin

Cat# 280301

Size : 50/pack

Brand : Biotoolomics


Introduction

Protein A SuperSpin is supplied for fast small-scale purification of immunoglobulin G (IgG) from various species (e.g. serum, ascites and mammalian cell culture broth etc). Protein A has been well proven a powerful affinity ligand which shows very high specificity to IgGs from mammalian hosts.

Each Protein A SuperSpin tube accommodates 50 µl of Protein A agarose particles. The affinity resin is specifically designed to provide large pore size with small particle size (i.e. 20 – 50 µm). So large antibody molecules can access the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantity of antibody can be easily purified within 20 minutes with inexpensive cost. No special expensive chromatography instrument and column is required.

    Protein A SuperSpin is particularly useful for the following applications:
  • The quantity of antibody to be purified is small (less than half milligram)
  • On-line detection of antibody expression level
  • High throughput purification of antibodies
  • High throughput screening of antibodies
    Key benefits:
  • Rapid antibody capture and recovery
  • High antibody binding capacity (half milligram)
  • High throughput format (24 samples can be processed in parallel)
  • Low cost
  • Easy to use and no expensive equipment required

Characteristics of Ni SuperSpin / Cu SuperSpin / Co SuperSpin / Zn SuperSpin

Product characteristics
Tube material Polypropylene
Resin Protein A SepFast
Particle size 20 – 50 µm
Packed volume 50 µl
Protein binding capacity Depends on the type of proteins and binding conditions;
up to 500 µg
Chemical compatibility  The resin maintains the binding capability after exposing to 3M NaSCN, 6M GuHCl or 8M urea over a few hours
Storage condition 2oC – 8oC

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