Xanthine Oxidase (XOD) Activity Assay Kit
Cat# E-BC-K805-M-96T
Size : 96T
Brand : Elabscience
| Detection Principle | Xanthine Oxidase (XOD) is mainly present in milk, liver and spleen of mammals, belonging to aerobic dehydrogenases, which is an important enzyme in nucleic acid metabolism in the body. When hepatocytes are injured, this enzyme is released into the serum earlier than SGPT, and increase its activity in serum significantly, which has obvious significance for the identification of hepatocellular jaundice and it is used for the identification of obstructive jaundice. In the process of hypoxia, xanthine dehydrogenase quickly forms xanthine oxidase, which plays an important role in free radical production. XOD can catalyze hypoxanthine to xanthine, and at the same time while producing superoxide anion free radicals. In the presence of electron acceptor and chromogenic agent, the purplish red substance can be generated, and the vitality of XOD can be calculated by measuring the production of purplish red substance. |
| Synonyms | XOD |
| Sample Type | Serum,Plasma,Animal tissue sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.067 U/L |
| Detection Range | 0.067-39.30 U/L |
| Precision | inter-assay CV: 9.9% | intra-assay CV: 0.03 |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rat plasma | 1 |
| Rat serum | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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