Citrate Synthase Activity Colorimetric Assay Kit

Citrate Synthase is a pace-making enzyme in the first step of the Citric Acid Cycle that catalyzes the conversion of acetyl-CoA and oxaloacetate into citrate and exists in all living organisms. Citrate synthase is localized in the mitochondrial matrix within eukaryotic cells and serves as a marker for intact mitochondria. The increase of mitochondrial citrate synthase activity is directly associated with endocrine and metabolic abnormalities such as obesity.

 The Citrate Synthase Activity Colorimetric Assay Kit provides a simple and convenient way for the quantification of citrate synthase activity in a variety of biological samples. In the assay, citrate synthase reacts with CS substrate mix to generate an intermediate, which subsequently reacts with CS developer to generate the colored product, which can be easily detected by plate reader or spectrophotometer at OD412 nm. The rate of color produce is proportional to citrate synthase activity. The assay is simple, convenient and rapid, and can detect the enzyme activity less than 1 mU in a variety of biological samples.