pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.

pNPP ONE liquid ready-to-use substrate is active and stable. PNPP ONE shows superior background characteristics due to its high temperature robustness compared to pNPP (cat. no. 4400).

The product is available in bulk quantities and as customized solutions.