Gain Consistency with our Single Lot Media and Reagents
CultureBoost™ is the broad-spectrum mitogen in Cell Systems Complete Medium (4Z0-500) and Cell Systems Medium Without Serum (4Z3-500). CultureBoost contains Cell Systems bovine growth factor and porcine heparin. One 5mL vial of CultureBoost™ supplements one 500mL bottle of media.
CultureBoost™ is purified from bovine pituitary or hypothalamus and is 100% traceable to cattle processed in the USA at USDA inspected facilities. The product's working concentration is 50µg/mL. Biological activity is adjusted to 110% of maximum using CSC 2V0 Human Venous Endothelial cells, ACBRI 181 Primary Retinal Microvascular Endothelial cells, and ACBRI 376 Primary Brain Microvascular Endothelial cells.
CultureBoost™ is qualified for use with Cell Systems media, and when initiating, thawing, expanding or passaging cultures of all mesenchymal-derived cells.
Use of CultureBoost™ can result in as much as 25% increase in recovery from freezing, an increase of 10-20% in cell count per passage, and a one day decrease in time-to-confluence. The effects of CultureBoost™ are most pronounced with extremely fastidious cells.
Available Formats
A 5mL vial is included in various Cell Systems media kits and in "The System™". CultureBoost™ 2x5mL vials are also available individually here.
Storage and Handling
This product is delivered at ambient temperature. Use 10ml to supplement 500ml cell culture medium. If the entire unit will not be used within 30 days, aliquot and freeze. Once opened, shelf life is 30 days at +2-8°C.
Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".