Reagents and instruments for immunology, cell biology and molecular biology.
 
> McCoy’s 5A Medium, Iwakata & Grace, w/L-Glutamine, w/o Sodium Bicarbonate (Powder)

McCoy’s 5A Medium, Iwakata & Grace, w/L-Glutamine, w/o Sodium Bicarbonate (Powder)

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Cat# M2702-50L

Size : 50L

Brand : US Biological


M2702 McCoy’s 5A Medium, Iwakata & Grace, w/L-Glutamine, w/o Sodium Bicarbonate (Powder)

Clone Type
Polyclonal
Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT/4°C

In 1959, McCoy and his coworkers reported the amino acid requirements for in vitro cultivation of Novikoff Hepatoma Cells. These studies were performed using Basal Medium 5A, and subsequently modified to create a new medium known as McCoy’s 5A Medium. This media has been employed to support the growth of primary cultures derived from adrenal glands, bone marrow (normal), gingiva, lung, mouse kidney, omentum, skin, spleen and other tissues. It is a general purpose medium for primary and established cell lines.

Applications
Appearance: Off-white to light orange, homogeneous, free flowing powder||Solubility: Yellow, clear, complete||pH: As Reported||Endotoxin: ≤1EU/ml||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. McCoy, T.A., Maxwell, M. and Kruse, P.F., Biol. Med. 100:55-118 (1959)|2. Patterson, M.K. and Dell’orco, R.T., Tissue Culture Association Manual.4:737-740 (1978)|3. Hsu, T.C. and Kellogg, D.S., J.N.C.I. 25:221-231 (1960)|4. Iwakata, S. and Grace, J.T., New York State Journal of Med. 64,:279-2282 (1964)|5. Morton, H.J., In Vitro. 6:89 (1970)|6. Culture Conditions and Types of Growth Media for Mammalian Cells, Zhanqiu Yang and Hai-Rong Xiong; http://dx.doi.org/10.5772/52301