TMB PLUS2

Cat# 4395L

Size : 500ML

Brand : Kementec


Three brown laboratory bottles in different sizes with Kementec Solutions TMB PLUS2-Prestained
Three brown laboratory bottles in different sizes with Kementec Solutions TMB PLUS2-Prestained and ELISA Plate Blocking
Graph illustrating sensitivity and speed og the different TMB formulations
Graph illustrating the stability of TMB PLUS2 over time
Electrochemical properties TMB PLUS2

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB PLUS2® characteristics are:

  • Medium sensitivity
  • Very low background
  • Aqueous based – no organic solvents (REACH Annex XVII compliant)
  • Low lot-to-lot variation
  • Suitable for use in electrochemical assays

For traceable pipetting, we recommend using pink colored TMB PLUS2® Prestained (cat. no. 4445). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.

Specifications

  • Available sizes: 100 mL, 500 mL, 1 L, bulk
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C in the dark
  • Stability: 4 years
  • Development time: 5-30 minutes
  • Stop solution: 0.2 M H₂SO₄

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Cat#
Description
Cond.
Price Bef. VAT
4395A
 1L 
4380L
 500ML 
4395H
 100ML