Lens culinaris (LCA/LCH)

Cat# 518472-50mg

Size : 50mg

Brand : US Biological



Shipping Temp
Blue Ice

Storage Temp
-20°C

LCA/LCH
Fractionated Lens culinaris lectin (LCA) is composed of four subunits that consists of an alpha-chain (MW: 5.7kD) and β-chain (MW: 17.5kD). It has a carbohydrate specificity toward alpha-D-mannose and alpha-D-glucose, eluting with the sugar MealphaMan+MealphaGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Source:
Lentil

Blood Specificity:
Non-specific

Sugar Specificity:
alpha-Mannose, alpha-Glucose

Inhibitory Carbohydrate:
alpha-Methylmannoside + alpha-Methylglucoside

Divalent Ions:
Ca++, Mn++

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Lentil

Purity
Semi-Pure

Form
Supplied as a lyophilized powder.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.