Lotus tetragonolobus Lectin (LTL)

Cat# L3445-10-5mg

Size : 5mg

Brand : US Biological



Grade
Molecular Biology Grade

Applications
E

Shipping Temp
Blue Ice

Storage Temp
-20°C

Asparagus Pea, Winged Pea
Lotus tetragonolobus lectin is a family of closely related glycoproteins having 2 or 4 subunits of about 28,000D. The isoelectric points differ somewhat but these isolectins probably have similar specificities toward alpha-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are markedly different between these fucose-specific lectins.

Applications:
User-Optimized.

Inhibiting/Eluting Sugar:
50mM-100mM L-fucose

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Lotus tetragonolobus seeds

Purity
2 bands by SDS-PAGE corresponding to the subunits of the

lectin

Concentration
Not determined

Form
Supplied as a lyophilized powder. Although many buffers can be employed for reconstituting and diluting L3445-10, 10mM HEPES buffered saline, pH 7.5 - 8.5, 0.1mM Ca++ is recommended. L3445-10 may be preserved with 0.04% sodium azide.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.