4800A | TMB X-tra Biotrend USA

Three brown laboratory bottles in different sizes with Kementec Solutions TMB X-tra-Prestained

Three brown laboratory bottles in different sizes with Kementec Solutions TMB X-tra-Prestained and ELISA Plate Blocking

Graph illustrating sensitivity and speed og the different TMB formulations

Graph illustrating the stability of TMB x-tra over time

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB X-tra characteristics are:

High sensitivity
Stable and robust
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Low lot-to-lot variation
Suitable for use in electrochemical assays

For traceable pipetting, we recommend using pink colored TMB X-tra Prestained (cat. no. 4810). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.