7α-Hydroxycholesterol [566-26-7]
Cat# HY-N7264-10mg
Size : 10mg
Brand : MedChemExpress
7α-Hydroxycholesterol is a cholesterol oxide and is formed by both enzymatic and non-enzymatic oxidation. 7α-Hydroxycholesterol can be used as a biomarker for lipid peroxidation.
For research use only. We do not sell to patients.
7α-Hydroxycholesterol Chemical Structure
CAS No. : 566-26-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Other Forms of 7α-Hydroxycholesterol:
- 7α-Hydroxycholesterol-d7 In-stock
Description |
7α-Hydroxycholesterol is a cholesterol oxide and is formed by both enzymatic and non-enzymatic oxidation. 7α-Hydroxycholesterol can be used as a biomarker for lipid peroxidation[1][2]. |
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Molecular Weight |
402.65 |
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Formula |
C27H46O2 |
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CAS No. |
566-26-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2(10)[C@]3(10)[C@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3(10)CC[C@]12C |
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Structure Classification |
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Initial Source |
fish oil, serum |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvent & Solubility |
In Vitro:
DMSO : 50 mg/mL (124.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.99% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.4835 mL | 12.4177 mL | 24.8355 mL | 62.0887 mL |
5 mM | 0.4967 mL | 2.4835 mL | 4.9671 mL | 12.4177 mL | |
10 mM | 0.2484 mL | 1.2418 mL | 2.4835 mL | 6.2089 mL | |
15 mM | 0.1656 mL | 0.8278 mL | 1.6557 mL | 4.1392 mL | |
20 mM | 0.1242 mL | 0.6209 mL | 1.2418 mL | 3.1044 mL | |
25 mM | 0.0993 mL | 0.4967 mL | 0.9934 mL | 2.4835 mL | |
30 mM | 0.0828 mL | 0.4139 mL | 0.8278 mL | 2.0696 mL | |
40 mM | 0.0621 mL | 0.3104 mL | 0.6209 mL | 1.5522 mL | |
50 mM | 0.0497 mL | 0.2484 mL | 0.4967 mL | 1.2418 mL | |
60 mM | 0.0414 mL | 0.2070 mL | 0.4139 mL | 1.0348 mL | |
80 mM | 0.0310 mL | 0.1552 mL | 0.3104 mL | 0.7761 mL | |
100 mM | 0.0248 mL | 0.1242 mL | 0.2484 mL | 0.6209 mL |
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
7α-Hydroxycholesterol566-26-7OthersCholesteroloxideenzymaticnon-enzymaticoxidationbiomarkerlipidperoxidationInhibitorinhibitorinhibit