AM4-65

Cat# 70039

Size : 1MG

Brand : Biotium


AM4-65

AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde.

Product Attributes

Probe cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Colors

Far-red

Excitation/Emission

510/750 nm (see product description)

Product Description

AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.

  • λExEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • MW: 844.85

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-65.

Nerve Terminal Dyes and Kits

Product Catalog No. Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)
70020... 70053 View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)
70021... 70050 View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-7 70029 Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS 70037 Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 101 80101 Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I 70030 Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A) 70031 Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B) 70031-1 Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III 70032 Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V 70034 Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
 

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