Anti-CCR2 (C-C Chemokine Receptor Type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, JE/FIC Receptor, MCP-1 Receptor, CD192, Cmkbr2) (APC) Monoclonal Antibody

Cat# C2099-66P-100T

Size : 100Tests

Brand : US Biological

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C2099-66P CCR2 (C-C Chemokine Receptor Type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, JE/FIC Receptor, MCP-1 Receptor, CD192, Cmkbr2) (APC)

Clone Type
Polyclonal
Host
rat
Source
mouse
Isotype
IgG2b
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors include monocytes, T cells, NK cells, basophils, mast cells, and dendritic cells.||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 10ul labels 1x10e6 cells.|Optimal dilutions to be determined by the researcher.||Hybridoma:|Fusion of a mouse myeloma with B cells obtained from a rat immunized with L1.2 cells transfected with mouse CCR2.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 10K31|Host: rat|Source: mouse|Concentration: ~0.01mg/ml|Form: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant.|Immunogen: L1.2 cells transfected with mouse CCR2.|Specificity: Recognizes mouse and rat CCR2 transfectants but not the parental cell line in Flow Cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in Flow Cytometry.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
L1.2 cells transfected with mouse CCR2.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse and rat CCR2 transfectants but not the parental cell line in Flow Cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in Flow Cytometry.
References
1. Apuzzo T, Bachi A, Bianchi ME, Celona B, De Marchis F, Livoti E, Mellado M, Munoz LM, Pedotti M, Proudfoot A, Raucci A, Schiraldi M, Thelen M, Uguccioni M, Varani L, Venereau E J. Exp. Med. 2012 209:551-63