Anti-CD19 (B4, Leu-12) (APC/Cy5.5) Monoclonal Antibody

CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro- B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody recognizes the same epitope as the published clone, 1D3.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 0.3ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy5.5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.