Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CD205 (WC6) (APC) Monoclonal Antibody

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Cat# 214609-APC-100ul

Size : 100ul

Brand : US Biological

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214609-APC CD205 (WC6) (APC)

Clone Type

Polyclonal

Host

mouse

Source

bovine

Isotype

IgG2b

Grade

Affinity Purified

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD205 cell surface, a 210-220kD molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.||Applications: |Suitable for use in Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|1:50-1:200|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2b|Clone No: CC98|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography.|Specificity: Recognizes bovine CD205. Species Crossreactivity: Sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes bovine CD205. Species Crossreactivity: Sheep

References

1. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle. Vet. Immunol. Immunopathol. 39: 25-48. 2. Howard, C.J. et al. (1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma/delta TCR+ T cells. Immunology 88: 558. 3. Naessens, J. et al. (1993) Cross reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 4. Gliddon, D. R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph. Immunology 111: 262-272. 5. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep. Dev Comp Immunol. 32: 837-49. 6. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification. Vet Res. 42:19. 7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848. PLoS One. 5: e13705. 8. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts. Viral Immunol. 20: 119-30. 9. Eicher, S.D. et al. (2011) ß-Glucan plus ascorbic acid in neonatal calves modulates immune functions with and without Salmonella enterica serovar Dublin. Vet Immunol Immunopathol. 142: 258-64. 10. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells PLoS One. 7: e30430. 11. Thonur, L. et al. (2012) Toll-like Receptor Gene Expression in Fresh and Archived Ovine Pseudoafferent Lymph DEC205(+) Dendritic Cells. J Comp Pathol. Apr 18. [Epub ahead of print] 12. Sigmundsdottir, H. et al. (2007) DCs metabolize sunlight-induced vitamin D3 to 'program' T cell attraction to the epidermal chemokine CCL27. Nat Immunol. 8: 285-93. 13. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts. Viral Immunol. 20: 119-30. 14. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells. J Histochem Cytochem. 54: 1021-30.