Anti-CD4 (L3T4, Ly-4) (APC/Cy5.5) Monoclonal Antibody

CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/ inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the monoclonal antibody effectively depletes CD4+ cells. It also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 0.2ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy5.5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.