Anti-CD44 (H-CAM, PGP-1) (PE) Monoclonal Antibody

Cat# 227422-100T

Size : 100Tests

Brand : US Biological

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227422 CD44 (H-CAM, PGP-1) (PE)

Clone Type
Polyclonal
Host
mouse
Source
equine
Swiss Prot
Q05078
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion.||Equine CD44 is widely expressed and may be used as a pan equine leucocyte marker.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CVS18|Host: mouse|Source: equine|Concentration: Not determined |Form: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE). |Purity: Purified by Protein G affinity chromatography.|Immunogen: Equine leucocytes.|Specificity: Recognizes equine CD44.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Equine leucocytes.
Form
Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes equine CD44.
References
1. Kydd, J.H. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Vet Immunol Immunopathol 42: 3 - 60. 2. Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1 J Gen Virol. 84: 293-300. 3. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 81: 312-23. 4. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations. Stem Cells Dev. 19: 269-82. 5. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells. Cell Medicine. 4: 1-11.