Figure-1: Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41 kDa.