Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Glutamine Synthetase, Microbial (Biotin) Polyclonal Antibody

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Cat# G7121-03-100ug

Size : 100ug

Brand : US Biological

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G7121-03 Glutamine Synthetase, Microbial (Biotin)

Clone Type

Polyclonal

Host

goat

Isotype

IgG

Grade

Highly Purified

Applications

E IP WB

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000-1:20,000. 1ug of Glutamine Synthetase was used in a standard capture ELISA with Streptavidin (HRP)( S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA and 0.01% sodium azide.|Purity: Purified by delipidation, salt fractionation and ion exchange chromatography prior to conjugation to biotin.|Immunogen: Glutamine Synthetase (microbail)|Specificity: Recognizes Glutamine Synthetase. Target species: microbial. Known reactivity: Brevibacterium. Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified Glutamine Synthetase.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Glutamine Synthetase (microbail)

Form

Supplied as powder lyophilized from a solution containing PBS (pH 7.2), 10mg/ml BSA and 0.01% sodium azide.

Purity

Purified by delipidation, salt fractionation and ion exchange chromatography prior to conjugation to biotin.

Specificity

Recognizes Glutamine Synthetase. Target species: microbial. Known reactivity: Brevibacterium. Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified Glutamine Synthetase.

References

Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.