Anti-Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Growth Factor 2, TCGF2) (PE) Monoclonal Antibody

Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which stimulates the growth and survivability of certain B cells and T cells. IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the TH2 type. TH2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled bu TH1 cells. The second is regulating IgE and IgG1 production by B cells. ||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.|Optimal dilutions to be determined by the researcher.||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. DO NOT FREEZE! For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.