Anti-Lamin B1 (Biotin) Monoclonal Antibody

The nuclear lamina consists of a layer of proteins which are in close apposition to the nucleoplasmic surface of the inner nuclear membrane. This structure provides a framework for nuclear envelope integrity, and for interphase chromosome organization. The lamina itself is composed of as many as four distinct lamin proteins which possess extensive structural similarities to intermediate filament proteins. Based on biochemical and structural properties, lamins can be classified into A- and B- type isoforms. Four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamins A and C are alternatively spliced products of a single gene, whereas lamins B1 and B2 are encoded by distinct genes. Differential lamin expression is thought to play a role in development and differentiation. Nuclear lamina in embryonic and lymphoid cells are composed exclusively of lamin B. ||Applications: |Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.||Recommended Dilutions:|Immunofluorescence: 5-10ug/ml|ELISA: 0.1-1.0ug/ml |Western Blot: 0.5-1.0ug/ml|Immunoprecipitation: 2.5-5ug|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.