Reagents and instruments for immunology, cell biology and molecular biology.

Anti-MHC Class 1, H2, Dd (Biotin) Monoclonal Antibody

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Cat# M3885-63B-500ug

Size : 500ug

Brand : US Biological

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M3885-63B MHC Class 1, H2, Dd (Biotin)

Clone Type

Polyclonal

Host

mouse

Source

mouse

Isotype

IgG2a,k

Grade

Purified

Applications

FC IP

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

This antibody has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.||Applications:|• Flow cytometry1|• Complement-dependent cytotoxicity1|• Blocking of antigen recognition|• Immunoprecipitation|• Immunoaffinity chromatography||Recommended Dilution:|Flow Cytometry: ≤ 1ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Mab|Isotype: IgG2a,k|Clone No: 34-5-8S|Host: mouse|Source: mouse|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.|Purity: Purified|Immunogen: C57BL/6 x DBA/2)F1 hybrid mouse splenocytes|Specificity: Reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

C57BL/6 x DBA/2)F1 hybrid mouse splenocytes

Form

Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.

Purity

Purified

Specificity

Reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.

References

1. Ozato, K., N. Mayer, and D.H. Sachs. 1980. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124:533-540.|2. Evans, G.A., D.H. Margulies, B. Shykind, J.G. Seidman, and K. Ozato. 1982. Exon shuffling: Mapping polymorphic determinants of mouse transplantation antigens. Transplantation 34:113-120.|3. Otten, G.R., E. Bikoff, R.K. Ribaudo, S. Kozlowski, D.H. Margulies, and R.N. Germain. 1992. Peptide and b2-microglobulin regulation of cell surface MHC class I conformation and expression. J. Immunol. 148:3723-3732.