Anti-Mouse CD4 (Clone GK1.5) - FITC
Cat# C213-100
Size : 100µg
Brand : Leinco Technologies
AntiMouse CD4 [GK1.5] – FITC
AntiMouse CD4 [GK1.5] – FITC
Product No.: C213
Clone GK1.5 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4mut, L3T4, Ly4, Cd4, CD4 Antigen Isotype IgG2b Applications FC , ICC , IHC FF |
Data
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This Fluorescein conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient Excitation Laser Blue Laser (490 nm) RRIDAB_2829211 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone GK1.5 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHCFF ICC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat AntiMouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for Tcell activation.1 Antigen Distribution The L3T4 antigen is expressed by the helper/inducer subset of mouse Tcells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned Tcell lines. Ligand/Receptor MHC class II molecule PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Hendrickson WA et al. (1994) Structure 2: 59 |