Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Sm (Smith) Antigen Polyclonal Antibody

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Cat# S1014-25-2ml

Size : 2ml

Brand : US Biological

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S1014-25 Sm (Smith) Antigen

Clone Type

Polyclonal

Host

human

Source

human

Isotype

IgG

Grade

Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE. ||Applications: |Suitable for use in ELISA and Immunodiffusion. Other applications not tested.||Recommended Dilutions: |ELISA: 1:100|Optimal dilutions to be determined by the researcher.||Titer:|Immunodiffusion: 1:2||Sm: Autoantibody Activity (by ELISA):|171.60EU/ml (lot-specific; value may differ from lot to lot).||Other Autoantibody Activity in the Serum (by ELISA):|SSA: 58.93EU/ml|SSB: 1.33EU/ml |SCL: 2.09EU/ml|Sm/RNP: 209.86EU/ml|Jo-1: 4.81EU/ml||Interpretation of Results:|Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: IgG|Host: human|Source: human|Concentration: Not Determined|Form: Supplied as a lyophilized powder from 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.|Purity: Purified by fractionation of delipidated and defibrinated donor human serum. |Immunogen: Sm(Smith). Autoantibody derived from human serum.|Specificity: Recognizes human Sm. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: RNP|and anti-SSA (low levels). No reactivity at a dilution of 1:100 when tested against purified SSB, Scl-70 and Jo-1 in an ELISA system designed to detect all IgG subclasses. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Sm(Smith). Autoantibody derived from human serum.

Form

Supplied as a lyophilized powder from 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.

Purity

Purified by fractionation of delipidated and defibrinated donor human serum.

Specificity

Recognizes human Sm. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: RNP|and anti-SSA (low levels). No reactivity at a dilution of 1:100 when tested against purified SSB, Scl-70 and Jo-1 in an ELISA system designed to detect all IgG subclasses.