Anti-Surfactant Protein D (SP­D, SFTPD) (PE) Monoclonal Antibody

Surfactant protein D (pSP-D), is a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs.||Applications: |Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry (paraffin): 1ug/ml, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections.|Optimal dilutions to be determined by the researcher.||Hybridoma: |P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Positive Control:|Porcine lung tissue||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.