Anti-TCR, gamma, delta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC) Monoclonal Antibody

Cat# 215154-FITC-100ul

Size : 100ul

Brand : US Biological

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215154-FITC TCR, gamma, delta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
chicken
Isotype
IgG1,k
Grade
Purified
Applications
IHC IP
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
-20°C

TCR gamma/delta is expressed on peripheral blood T cells. In the chicken TCR g/d is more widely expressed than in mammals, approximately 20-25% of total blood T cells being gamma/delta +ve , in young birds (age around 6 months) this figure can rise to nearly 50%.||Applications: |Suitable for use in Flow Cytometry (Not Tested), Immunoprecipitation and Immunohistochemistry (frozen). Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 10ul labels 1x10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: TCR-1|Host: mouse|Source: chicken|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Ion Exchange chromatography from tissue culture supernatant.|Immunogen: Outbred chicken thymocytes and Ig-negative blood lymphocytes|Specificity: Recognizes chicken TCR Gamma/Delta. Species Crossreactivity: milk snake||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Outbred chicken thymocytes and Ig-negative blood lymphocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Ion Exchange chromatography from tissue culture supernatant.
Specificity
Recognizes chicken TCR Gamma/Delta. Species Crossreactivity: milk snake
References
1. Sowder, J.T. et al. (1988) A large subpopulation of avian T cells express a homologue of the mammalian T gamma/delta receptor. J Exp Med. 167: 315­22.