Anti-Tissue Inhibitor of Metalloproteinases 3 (TIMP Metallopeptidase Inhibitor 3, TIMP3, TIMP-3) (FITC) Polyclonal Antibody

TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.||Applications:|Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 0.5-1ug/10e6 cells|Immunofluorescence: 1-2ug/ml|Western Blot: 0.5-1ug/ml|Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT|Immunoprecipitation: 1-2ug/500ug protein lysate|Optimal dilutions to be determined by the researcher.||Positive Control:|Placenta or breast carcinoma||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.