Reagents and instruments for immunology, cell biology and molecular biology.

Anti-TLR4 (Toll Like Receptor 4, CD284) (PE) Monoclonal Antibody

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Cat# T8050-34D-100T

Size : 100Tests

Brand : US Biological

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T8050-34D TLR4 (Toll Like Receptor 4, CD284) (PE)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

O00206

Isotype

IgG2a

Grade

Affinity Purified

Applications

Crossreactivity

Bo Ca Gp Hu Mk Po

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested. ||Recommended Dilutions:|Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. |Optimal dilutions to be determined by the researcher.| |Hybridoma: |Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: 3F5 (HTA125)|Host: mouse|Source: human|Concentration: Not Determined |Form: Supplied as a liquid in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). |Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ba/F3 cell line expressing TLR4.|Specificity: Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Rhesus monkey, guinea pig, porcine, canine and and bovine. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Ba/F3 cell line expressing TLR4.

Form

Supplied as a liquid in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Rhesus monkey, guinea pig, porcine, canine and and bovine.

References

1. Shimazu, R. et al. (1999). MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll like receptor 4. J. Exp. Med. 189: 1777.2. Jiang, Q. et al. (2000)