ATP4A (NM_000704) Human Tagged ORF Clone

CAT#: RC211772

ATP4A (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

Datasheet
SDS
Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ATP4A
Synonyms ATP6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211772 representing NM_000704
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGGCCGAGAACTATGAGCTCTACTCGGTGGAGCTGGGTCCTGGCCCTGGCGGGGACATGGCTG
CCAAGATGAGCAAGAAGAAGAAGGCGGGTGGCGGGGGTGGCAAGAGGAAGGAGAAGCTGGAGAACATGAA
GAAGGAGATGGAGATTAACGACCACCAGCTGTCAGTGGCGGAGCTGGAACAGAAATACCAGACCAGTGCC
ACCAAGGGCCTCTCTGCGAGCCTGGCTGCTGAGCTGCTGCTGCGGGATGGGCCCAACGCACTGCGGCCAC
CACGGGGCACCCCAGAGTACGTCAAGTTTGCGAGGCAGCTGGCCGGGGGCCTGCAGTGCCTCATGTGGGT
TGCCGCCGCCATCTGCCTCATCGCCTTTGCCATCCAGGCTAGTGAGGGGGACCTCACCACCGACGACAAT
CTGTACCTGGCAATCGCTCTCATTGCTGTGGTTGTCGTCACCGGCTGCTTTGGCTACTACCAGGAATTCA
AGAGCACCAACATCATCGCCAGCTTTAAGAACCTTGTGCCACAGCAAGCCACTGTCATCCGCGATGGAGA
CAAATTCCAGATCAACGCTGACCAACTGGTGGTGGGCGACCTGGTGGAGATGAAAGGTGGGGACAGAGTG
CCCGCCGACATCCGCATCCTGGCGGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTGACAGGGGAGTCTG
AGCCACAGACCCGCTCACCCGAGTGCACGCACGAGAGCCCTCTGGAGACCCGCAACATCGCCTTCTTCTC
CACCATGTGCCTTGAGGGCACCGTGCAGGGCCTGGTGGTGAACACGGGCGACCGCACCATCATTGGGCGC
ATCGCATCGCTGGCGTCGGGGGTGGAAAACGAGAAGACACCCATCGCTATCGAGATCGAGCATTTTGTGG
ACATCATCGCGGGCCTGGCCATTCTCTTCGGTGCCACATTTTTTATTGTGGCCATGTGCATTGGCTACAC
CTTCCTGCGGGCCATGGTCTTCTTCATGGCCATCGTGGTGGCCTATGTGCCTGAGGGGCTGCTGGCCACT
GTCACAGTCTGCCTGTCCCTGACAGCCAAGCGCCTGGCCAGTAAGAACTGCGTGGTCAAGAACCTGGAGG
CGGTGGAGACATTGGGCTCCACTTCGGTGATCTGCTCGGACAAGACAGGGACTCTCACTCAGAACCGCAT
GACTGTGTCCCATCTGTGGTTTGACAACCACATCCACACAGCTGACACCACGGAAGACCAGTCAGGGCAG
ACGTTTGACCAGTCCTCGGAGACGTGGCGGGCGCTGTGCCGGGTGCTCACCCTGTGCAACCGCGCCGCCT
TCAAGTCCGGCCAGGATGCAGTGCCTGTGCCCAAGCGCATCGTGATTGGAGACGCATCGGAGACGGCGCT
GCTCAAGTTCTCGGAGCTGACGCTGGGCAACGCCATGGGCTACCGGGACCGCTTCCCAAAAGTCTGCGAG
ATACCCTTCAACTCCACCAACAAGTTCCAGCTGTCCATCCATACGCTGGAGGACCCGCGGGACCCGCGAC
ACTTGCTGGTGATGAAGGGCGCCCCCGAGCGCGTGCTGGAGCGCTGCAGCTCCATCCTTATCAAGGGCCA
GGAGCTGCCGCTGGACGAGCAGTGGCGCGAGGCCTTCCAGACCGCCTACCTCAGCCTGGGAGGCCTGGGC
GAACGCGTGCTCGGCTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCGCCTGGCTATGCCTTCGACG
TAGAGGCCATGAACTTTCCATCTAGCGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCACCCCG
GGCCACCGTCCCTGATGCTGTGCTCAAGTGTCGCACCGCAGGCATCCGGGTGATCATGGTAACGGGTGAC
CACCCCATCACCGCCAAGGCCATTGCAGCCAGTGTGGGCATCATCTCGGAAGGCAGCGAGACAGTGGAGG
ACATCGCTGCCCGCCTCCGTGTGCCCGTAGACCAGGTTAATCGCAAGGATGCCCGTGCCTGTGTGATCAA
TGGCATGCAGCTGAAGGACATGGACCCATCGGAACTGGTCGAGGCCCTGCGCACCCACCCCGAGATGGTG
TTTGCGCGCACCAGCCCCCAGCAGAAGCTGGTGATCGTGGAGAGCTGCCAGCGGCTGGGTGCGATTGTGG
CCGTCACGGGGGATGGTGTGAATGACTCCCCAGCTCTGAAGAAGGCAGACATCGGAGTAGCCATGGGCAT
CGCTGGCTCAGATGCTGCCAAAAATGCAGCTGACATGATCCTGCTGGATGACAACTTTGCCTCCATTGTG
ACAGGCGTGGAGCAGGGTCGACTGATCTTCGACAACCTGAAGAAGTCTATTGCCTACACATTGACCAAGA
ACATCCCAGAGCTGACACCCTACCTCATCTACATCACCGTCAGCGTGCCCCTGCCCCTCGGGTGCATCAC
CATCCTCTTCATCGAACTCTGCACTGACATTTTCCCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGT
GACATCATGCACCTGCGTCCACGCAACCCAAAGCGTGACAGATTGGTCAACGAGCCCCTGGCTGCCTACT
CCTACTTCCAGATTGGTGCCATTCAGTCCTTTGCTGGCTTCACTGACTACTTCACGGCAATGGCCCAGGA
GGGCTGGTTCCCACTGCTGTGCGTGGGGCTGCGGGCGCAGTGGGAGGACCACCACCTACAAGATCTGCAG
GACAGCTACGGCCAGGAGTGGACATTCGGGCAGCGCCTGTACCAGCAGTACACCTGCTACACCGTGTTCT
TCATCAGCATTGAGGTGTGCCAGATCGCCGATGTCCTCATCCGCAAGACGCGCCGTCTCTCTGCCTTCCA
GCAAGGCTTCTTCAGGAATAAGATCCTGGTGATCGCCATCGTGTTCCAGGTCTGCATCGGCTGCTTCCTG
TGCTACTGCCCCGGCATGCCCAACATCTTCAACTTCATGCCCATTCGGTTCCAGTGGTGGCTGGTCCCCC
TGCCCTACGGCATCCTCATCTTCGTCTATGATGAGATCCGGAAGCTTGGAGTTCGCTGTTGCCCAGGGAG
CTGGTGGGACCAGGAACTCTACTATAGCGGACCG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
>RC211772 representing NM_000704
Red=Cloning site Green=Tags(s)

MGKAENYELYSVELGPGPGGDMAAKMSKKKKAGGGGGKRKEKLENMKKEMEINDHQLSVAELEQKYQTSA
TKGLSASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDN
LYLAIALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRV
PADIRILAAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTVQGLVVNTGDRTIIGR
IASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLAT
VTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQ
TFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCE
IPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLG
ERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGD
HPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMV
FARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIV
TGVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAES
DIMHLRPRNPKRDRLVNEPLAAYSYFQIGAIQSFAGFTDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQ
DSYGQEWTFGQRLYQQYTCYTVFFISIEVCQIADVLIRKTRRLSAFQQGFFRNKILVIAIVFQVCIGCFL
CYCPGMPNIFNFMPIRFQWWLVPLPYGILIFVYDEIRKLGVRCCPGSWWDQELYYSGP

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000704
ORF Size 3114 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000704.2, NP_000695.2
RefSeq Size 3582 bp
RefSeq ORF 3108 bp
Locus ID 495
UniProt ID P20648
Cytogenetics 19q13.12
Protein Families Druggable Genome, Transmembrane
Protein Pathways Oxidative phosphorylation
MW 113.9 kDa
Gene Summary The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Other Versions

SKU Description Size
RC211772L1 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged
    • 10 ug

1 204,00 EUR

RC211772L2 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged
    • 10 ug

1 204,00 EUR

RC211772L3 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged
    • 10 ug

1 204,00 EUR

RC211772L4 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged
    • 10 ug

1 204,00 EUR

RG211772 ATP4A (tGFP-tagged) - Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
    • 10 ug

1 104,00 EUR

SC300122 ATP4A (untagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
    • 10 ug

904,00 EUR