Caprine TNF alpha ELISA
Cat# DIY1457G-004
Size : 1Set
Brand : Kingfisher Biotech
Caprine TNFα ELISA Data
Caprine TNFα ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Ovine TNFα Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1454V-100 |
| Biotinylated Anti-Ovine TNFα Polyclonal Antibody | Detection Antibody | 50 µg | KPB1455V-050 |
| Caprine TNFα Recombinant Protein | Standard | 5 µg | RP1036G-005 |
Caprine TNFα ELISA Specifications
The Caprine TNFα (TNFSF2) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Caprine TNFα ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Background
TNFα (TNFSF2) is a member of the TNF Superfamily. It is produced chiefly by activated macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue. The primary role of TNFα is in the regulation of immune cells. TNFα, being an endogenous pyrogen, is able to induce fever, to induce apoptotic cell death, to induce sepsis (through IL-1 & IL-6 production), to induce cachexia, induce inflammation, and to inhibit tumorigenesis and viral replication.
Alternate Names - TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-α, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha