Caspase 5 (Caspase-5, CASP 5, CASP5, CASP-5, ICERELIII, ICErel-III, Ich3, Ich-3 Protease, TY, TY Protease) (MaxLight 550)

Cat# C2087-24Y-ML550-100ul

Size : 100ul

Brand : US Biological



C2087-24Y-ML550 Caspase 5 (Caspase-5, CASP 5, CASP5, CASP-5, ICERELIII, ICErel-III, Ich3, Ich-3 Protease, TY, TY Protease) (MaxLight 550)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
P51878
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.||Caspase-5, a 47kD cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE­type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1). ||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1-2ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.|Purity: Purified by affinity chromatography.|Immunogen: Synthetic peptide corresponding to 14aa from the center of human Caspase-5. |Specificity: Recognizes human Caspase-5.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to 14aa from the center of human Caspase-5.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Caspase-5.
References
1. Wang, S. et al. (1996) Identification and characterization of Ich-3, a member of the Interleukin-1beta converting enzyme (ICE)/Ced­3 family and an upstream regulator of ICE. J. Biol. Chem. 271: 20580­20587. 2. Lin, X.Y. et al. (2000) Expression analysis of the human Caspase-1 subfamily reveals specific regulation of the CASP5 gene by Lipopolysaccharide and Interferon­gamma. J. Biol. Chem. 275: 39920­39926. 3. Munday, N.A. et al. (1995) Molecular cloning and pro­apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED­3 family of cysteine proteases. J. Biol. Chem. 270: 15870­15876. |4. Cohen, G.M. (1997) Caspases: the executioners of apoptosis. Biochem. J. 326: 1­16. Storage Store at +4oC or at ­20oC if preferred.