CheKine™ Micro Hexokinase (HK) Activity Assay Kit
Cat# KTB1123-96
Size : 96T
Brand : Abbkine
Specification
| Product name | CheKine™ Micro Hexokinase (HK) Activity Assay Kit |
| Applications notes | CheKine™ Micro Hexokinase (HK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm |
Product Properties
| Kit components | •Extraction Buffer • Assay Buffer • Substrate Mix • Enzyme Mix • WST-8 • Enhancer • NADH Standard |
| Features & Benefits | • Detect hexokinase (HK) activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells • Detailed sample preparation and results calculation methods are provided |
| Calibration range | 31.25-2000μM |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • It is advisable to measure serum potassium as soon as possible after blood collection. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Hexokinase (HK) is widely found in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose to glucose 6-phosphoglucose, which is the intersection of glycolysis and pentose phosphate pathways |
Image & description
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Theaflavin 3, 3′-digallate stabilizes vulnerable plaques by reprogramming metabolic homeostasis in neovascularization via HK2/TIGAR.(KTB1123-8.300)
Author：Cen, Kuan, et al. Publication name： Phytomedicine IF：8.300
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Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation.(KTB1123-4.900)
Author：Sun, Zhe, et al. Publication name：PLoS pathogens IF：4.900
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