Fms, Recombinant, Human, GST-Tag (CD115, c-fms, CSF-1R, M-CSFR)

Human FMS (GenBank Accession No. BC047521), (aa538-end) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. ||Specific Activity: |300U/ug. ||Unit Definition:|One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C. ||Assay Buffer: |50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate, and 1ug/ml recombinant FMS. ||Applications: |Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.