Glycerol is a polyol and acts as a backbone to all lipids known as triglycerides. Glycerol is widely used in solvents, beverages, foods, pharmaceutical and cosmetic products, etc. Quantification of glycerol causes broad interest for research and development.

The Free Glycerol Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of free glycerol in various samples based on colorimetric and fluorometric method. In the assay, glycerol is enzymatically oxidized to produce a product which reacts with the probe to yield fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The kit can detect 50 pmol-10 nmol (or 1-10000 µM range) glycerol in various samples.