Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat anti-Patched 1 (zebrafish)

Goat anti-Patched 1 (zebrafish)

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Cat# EB07960

Size : 100ug

Brand : Everest Biotech


Code Name Applications Availability Size Tested species Grade
EB07960 Goat Anti-Patched 1 (zebrafish) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Zebrafish

Target Protein

Principal Names: ptc1, patched 1, ptc-1, etID309849.2, patched1
Official Symbol: ptc1
Accession Number(s): NP_571063.1

Immunogen

Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25;8:40.; PMID: 19555497). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Zebrafish
Expected from sequence similarity: Zebrafish

Specific Reference

This antibody has been successfully used (in IF on Zebrafish tumors) in the following paper:
Ju B, Spitsbergen J, Eden CJ, Taylor MR, Chen W.
Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish.
Mol Cancer. 2009 Jun 25;8:40.
PMID: 19555497