Griffonia simplicifolia GS II (Bandeiraea simplicifolia) (Biotin)

This lectin is a tetrameric glycoprotein composed of four subunits of nearly identical size. Each 30,000D subunit has a binding site for alpha- or beta-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively alpha- or beta-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Chitin Hydrolysate or 200mM N-acetylglucosamine

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Griffonia (Bandeiraea) simplicifolia seeds

Not determined

Supplied as a lyophilized powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Reconstitute with 1ml dH2O. Labeled with Biotin.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.