| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptide (SAGIINYQRRTTSS - C) of human keratin K1 (formerly also designated cytokeratin 1), coupled to KLH |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P04264 (Human),Q99PS0 (Mouse) |
| Synonym | Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum)!
Polypeptide reacting: basic human keratin K1 (Mr 66,000).
Positive control: human foot sole.
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