Evercyte’s human colon epithelial cell line HCEC-1CT can be grown without limitations while maintaining expression of cell type specific markers and functions. Therefore, these cells are useful for setting up in vitro bioassays to study drug / nutrient absorption and to assess drug-induced toxicity on the large intestine barrier. Additionally, the cells are valuable for modelling cancer progression in vitro.
General information
Cat#: CkHT-039-0229
Morphology and expression of marker proteins
HCEC-1CT cell line shows the typical epithelial, cobblestone appearance and expression of characteristic, colonic epithelial markers such as A33, Mucin-1 as well as moderate expression of Villin in 2D differentiated cultures. Cell nuclei are counterstained with DAPI.
Cell-cell contacts in 3D spheroid culture
When grown as 3D spheroids, HCEC-1CT cells form multicellular organoid structures that stain positive for ZO1 demonstrating formation of cell-cell contacts. Cell nuclei are counterstained with DAPI.
FAQs
In vitro propagation
ColoUp (Evercyte, Cat# MHT-039)
DMEM (Gibco, Cat # 61965-026)/M199 (Gibco, Cat# 31150022) (4+1)
2 % Cosmic Calf Serum (Hyclone, Cat# SH30087)
20 ng/ml EGF (Sigma Aldrich, Cat# E9644)
10 µg/ml Insulin (Sigma Aldrich, Cat# I9278)
2 µg/ml Apo-Transferrin (Sigma Aldrich, Cat# T2036)
5 nM Sodium-Selenit (Sigma-Aldrich, Cat# S5261)
Additional material & reagents
Phosphate buffered saline (PBS) (Sigma, Cat# D8537)
Trypsin inhibitor (Gibco, Cat# R007100)
0,05 % Trypsin-EDTA (Gibco, Cat#25300-054)
Passaging of cells
For detachment of the cells remove and discard the culture medium and wash the cells once with PBS (about 160 µl/cm²). Remove PBS completely.
Cryopreservation
Freezing medium
ColoUp growth medium (Evercyte, Cat# MHT-039)
10% DMSO (Sigma Aldrich, Cat# D2650)
10% Cosmic Calf Serum (Hyclone, Cat# SH30087)
Additional material & reagents
0,05 % Trypsin-EDTA (Gibco, Cat# 25300-054)
Licence Conditions
The business concept of Evercyte is to out-license telomerized cells to our customers. The license conditions depend on whether the contract partner is a for profit or a nonprofit organization and the intended use of the cells.
Nonprofit organizations
On time payment for unlimited use: EUR 1700
Profit organizations
Pharmaceutical – chemical – cosmetic industries
Contract research organizations (CRO)
Initial license fee for 3 months: EUR 2700Annual license fee R&D: royalty based
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