Human CYCS (Cytochrome c) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	CYCS
Gene ID:	54205
Uniprot ID:	CYC_HUMAN
Specificity:	This kit recognizes Human Cyt-C in samples. No significant cross-reactivity or interference between Human Cyt-C and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Binds 1 heme c group covalently per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Protein Name	Cytochrome C
Database Links	Reactome: R-HSA-111457 Reactome: R-HSA-111458 Reactome: R-HSA-111459 Reactome: R-HSA-111463 Reactome: R-HSA-111464 Reactome: R-HSA-2151201 Reactome: R-HSA-3299685 Reactome: R-HSA-5620971 Reactome: R-HSA-5628897 Reactome: R-HSA-611105 Reactome: R-HSA-9627069 Reactome: R-HSA-9707564
Cellular Localisation	Mitochondrion Intermembrane Space Loosely Associated With The Inner Membrane
Alternative CLIA Names	Cytochrome C CLIA kit CYCS CLIA kit CYC CLIA kit
Specificity	This kit recognizes Human Cyt-C in samples. No significant cross-reactivity or interference between Human Cyt-C and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-114	104
EDTA plasma(n=8)	93-107	99
Cell culture media(n=8)	87-98	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	182.06	586.24	1766.38	184.30	557.14	1923.43
Standard deviation	19.06	69.12	138.48	23.46	57.00	160.80
CV (%)	10.47	11.79	7.84	12.73	10.23	8.36

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance