Human DEFA1; DEFA1B (Neutrophil defensin 1) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	DEFA1.DEFA1B
Gene ID:	1667/728358
Uniprot ID:	DEF1_HUMAN
Specificity:	This kit recognizes Human DEF Alpha 1 in samples. No significant cross-reactivity or interference between Human DEF Alpha 1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Effector molecule of the innate immune system that acts via antibiotic-like properties against a broad array of infectious agents including bacteria, fungi, and viruses or by promoting the activation and maturation of some APCs. Interacts with the essential precursor of cell wall synthesis lipid II to inhibit bacterial cell wall synthesis. Inhibits adenovirus infection via inhibition of viral disassembly at the vertex region, thereby restricting the release of internal capsid protein pVI, which is required for endosomal membrane penetration during cell entry. In addition, interaction with adenovirus capsid leads to the redirection of viral particles to TLR4 thereby promoting a NLRP3-mediated inflammasome response and interleukin 1-beta (IL-1beta) release. Induces the production of proinflammatory cytokines including type I interferon (IFN) in plasmacytoid dendritic cells (pDCs) by triggering the degradation of NFKBIA and nuclear translocation of IRF1, both of which are required for activation of pDCs.
Protein Name	Neutrophil Defensin 1 Defensin - Alpha 1 Hnp-1 Hp-1 Hp1 Cleaved Into - Hp 1-56 - Neutrophil Defensin 2 Hnp-2 Hp-2 Hp2
Database Links	Reactome: R-HSA-1461973 Reactome: R-HSA-1462054 Reactome: R-HSA-6798695
Cellular Localisation	Secreted
Alternative ELISA Names	Neutrophil Defensin 1 ELISA kit Defensin - Alpha 1 ELISA kit Hnp-1 ELISA kit Hp-1 ELISA kit Hp1 Cleaved Into - Hp 1-56 - Neutrophil Defensin 2 ELISA kit Hnp-2 ELISA kit Hp-2 ELISA kit Hp2 ELISA kit DEFA1 ELISA kit DEF1 ELISA kit DEFA2 ELISA kit MRS.DEFA1B ELISA kit
Specificity	This kit recognizes Human DEFα1 in samples. No significant cross-reactivity or interference between Human DEFα1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-97	91
EDTA plasma (n=8)	93-107	99
Cell culture media (n=8)	94-111	101

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	98.61	179.14	867.81	97.17	195.46	814.62
Standard deviation	6.80	8.12	40.79	6.08	11.00	43.66
CV (%)	6.90	4.53	4.70	6.26	5.63	5.36

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance