Human IgA + IgG + IgM Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Human IgG, IgA and IgM whole molecules
Specificity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA, Human IgG and Human IgM. This reagent is suitable for the detection of all human immunoglobulin subclases, isotypes and chain combinations.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.5 moles TRITC per mole of Goat IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity chromatography
Conjugation	TRITC
Storage	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.