Human IgD Mouse Monoclonal Antibody [Clone ID: NI 158]

Specifications

Product Data
Clone Name	NI 158
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgD, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified polyclonal IgD isolated from human serum.
Specificity	The reactivity of the antiserum is restricted to an isotype specific determinant of the IgD molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins. Label: Biotin State: Lyophilized purified Ig fraction Molar radio: Biotin/IgG ~5.0
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water.
Concentration	0.4 mg/ml
Conjugation	Biotin
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.