Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]

Specifications

Product Data
Clone Name	NI 330 (HP 6050)
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/250.  ELISA: from 1/1,000 upwards.  Western blot: from 1/500 upwards.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified monoclonal IgG3 isolated from pooled Human serum.
Specificity	The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Cross-Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized purified IgG fraction Molar radio: Biotin/IgG ~2.2
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	0.4 mg/ml
Conjugation	Biotin
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.